Key to Families (and Subfamilies) of Plant and Soil Nematodes

Rev. 10/14/08

1 a Nematodes with a protrusible stylet (stomatostyle) or spear (odontostyle) go to 2
b Nematodes without protrusible stylet or spear go to 24
2 a Stylet and three-part esophagus with median bulb (metacorpus), males usually with bursa go to 3
b Spear and two-part esophagus with posterior swollen (postcorpus), males usually without bursa but with supplements    go to 16
3 a Esophageal glands overlap intestine go to 4
b Esophageal glands enclosed in bulb that abuts intestine go to 9
4 a Cephalic framework well-developed, lip-region high. Females with two gonads go to 5
b Posterior gonad reduced to a post-uterine sac, vulva at 70-90%, lip-region flattened or cephalic framework absent  go to 8
5 a Tail tip, anus and vulva in close proximity on posterior of swollen female, male body twisted, male without bursa go to 6
b Anus and vulva anterior to tail tip even when female swollen, male body not twisted, male with bursa go to 7
6 Juvenile and female stages are sedentary endoparasites, female greatly swollen; male without bursa, body twisted, migratory in soil

i)  Body of dead female forms cyst and retains some or all eggs Heteroderinae
ii)  Female body does not form cyst, eggs deposited in matrix
Meloidogyninae
iii)  Only adult female swollen and sedentary, body does not form cyst, eggs deposited in matrix, male body twisted 
Nacobboderinae

Heteroderidae
       
7 a Stylet 2-3 x stylet body diameter, tail < 2 x anal body diameter, body spiral when heat-killed, vulva around 60% 

i)  Mature female remains vermiform  Hoplolaiminae 

ii)  Mature female saccate sedentary semi-endoparasite Rotylenchulinae

Hoplolaimidae
b Tail > 2.5 x anal body diameter, phasmids on tail region 

i)  Long nematodes, stylet > 3 x stylet body diameter Belonolaiminae                            
ii)  Stylet < 1.5 x stylet body diameter 
Telotylenchinae

Belonolaimidae  
8 a Lip-region flattened but cephalic framework well-developed 

i)  Female remains vermiform Pratylenchinae 

ii)  Female a saccate endoparasite Nacobbinae

Pratylenchidae
b Cephalic-framework weak or absent Anguinidae
9 a Females with two gonads, males with caudal bursa  Dolichodoridae
b Posterior female gonad reduced to a post-uterine sac, males with adanal bursa (if present) go to 10
10 a Median bulb large and refractive, stylet knobs absent or small go to 11
b Median bulb not refractive, stylet knobs obvious go to 12
11 a Stylet without knobs, tail rounded, male with bursa  Aphelenchidae
b Stylet with small knobs, tail pointed, male usually without bursa   Aphelenchoididae
12 a Stylet short (< stylet body diameter), tail pointed to filiform go to 13
b Stylet long so that knobs are close to median bulb valve, procorpus swollen and fused with metacorpus go to 14
13 a Tail long and filiform (>5 x anal body diameter) Tylenchidae
b Tail pointed (<5 x anal body diameter), cephalic framework absent  Anguinidae
14 a Thick cuticle with coarse striations go to 15
b i)  Female swollen, excretory pore posterior on body, female a saccate sedentary semi-endoparasite Tylenchulinae   
ii)  Cuticle with fine striations, male often without stylet, body C-shaped     
Paratylenchinae
Tylenchulidae
15 a i)  Large body diameter, sausage shape, slow moving, anchor-shaped stylet knobs             Criconematinae
ii)  Extra cuticular sheath, pointed tail, rounded stylet knobs     
Hemicycliophorinae
Criconematidae
16 a Spear with complex configuration, loose-fitting cuticle Diptherophoridae
b Spear simple, cuticle not loose but may be swollen go to 17
17 a Spear dorsally curved, anus almost terminal Trichodoridae
b Spear straight, anus not terminal - tail present go to 18
18 a Long, slender nematodes (1-12 mm), spear > 50 µm, long spear extension (odontophore) Longidoridae
b Short spear go to 19
19 a Postcorpus of esophagus with muscular sheath Belondiridae
b No muscular sheath around esophagus go to 20
20 a Spear extension (odontophore) thick and curved  Leptonchidae
b Spear extension not curved  go to 21
21 a Plump nematodes, tail rounded go to 22
b Slender nematodes, tail conoid to filiform go to 23
22 a Spear slightly curved, small opening Nordiidae
b Spear with large opening, cuticle appears layered at tail Aporcelaimidae
23 a Large nematodes (>2 mm), tail filiform (may be rounded in male) Thornenematidae
b Medium size (1-2 mm), tail conoid, body generally curved Qudsianematidae
24 a Body asymmetrical, caterpillar-like Bunonematidae
b Body symmetrical go to 25
25 a Median bulb present, tail filiform Diplogasteridae
b Median bulb absent go to 26
26 a Esophagus with terminal bulb or swelling go to 27
b Esophagus cylindrical, no swellings go to 37
27 a Terminal bulb without valves go to 28
b Terminal bulb with valves go to 29
28 a Cuticle not striated Monhysteridae
b Cuticle clearly striated  Bastianiidae
29 a Aquatic forms;  cuticle striated and punctated  Chromadoridae
b Cuticle without punctations go to 30
30 a Stoma cavity with teeth, tail filiform Diplogasteridae
b Stoma cavity without teeth go to 31
31 a Head with labial extensions or crown go to 32
b Head without extensions go to 34
32 a Head with lateral cuticular extensions Plectidae
b Head with anterior extensions only go to 33
33 a Labial extensions crown-shaped Teratocephalidae
b Labial-extensions thorn- or antler-shaped Cephalobidae
34 a Tail tip with spinneret, amphids easily visible, males with tubular supplements Plectidae
b Tail tip without spinneret go to 35
35 a Stoma cavity cylindrical, cuticular walls, males with bursa Rhabditidae
b Stoma cavity funnel-shaped, walls not cuticular, posterior female gonad reduced, anterior gonad reflexed and extending beyond vulva go to 36
36 a Stoma cavity clearly present Panagrolaimidae
b Stoma cavity difficult to see, only anterior section open Cephalobidae
37 a Cuticle striated Bastianiidae
b Cuticle smooth  go to 38
38 a Slender nematodes, no obvious stoma cavity or setae Alaimidae
b Clear stoma cavity or setae go to 39
39 a Amphids round, clearly visible Monhysteridae
b Amphids difficult to see, not round go to 40
40 a  Stoma cavity large, walls cuticular walls go to 41
b Stoma cavity funnel-shaped or not obvious, walls not cuticular go to 42
41 a  Tail filiform, setae present  Prismatolaimidae
b Tail not filiform, large stoma cavity with teeth  Mononchidae
42 a Funnel-shaped stoma with small teeth Tobrilidae
b Stoma closed anteriorly, one tooth in lumen Tripylidae

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March 1996

Howard Ferris                           Key modified from:    Tom Bongers
Department of Nematology                                         Wageningen University
University of California                                                Wageningen
Davis, CA 95616                                                        The Netherlands