NEMATOLOGY 100
Laboratory Exercise 16
Rev. 12/03/2008
Purpose: Rhizosphere and soil biology in relation to nematode ecology and crop production.
1. Slide presentation: Soil Biology and Crop Production
2. Examine the various nematode
trapping fungi, including those with:
adhesive mycelial networks (e.g., Monacrosporium eudermatum, Arthrobotrys oligospora);
adhesive knobs (e.g., Monacrosporium ellipsosporum);
adhesive hyphae (e.g., Monacrosporium cyanopagum,
M. gephyrapagum);
constricting ring traps (e.g., Arthrobotrys dactyloides);
sticky
spores (e.g., Meria coniospora
and Hirsutella rhossiliensis).
Examine the fungi and trap mechanisms in the petri dish - you may need to invert
the dish and focus through the agar.
Some plates seeded with nematodes last week.. Meloidogyne J2 were added 16 hours ago. A mixture of nematodes is available if you want to add more. (*****Best to use Heterorhabditis for this demo****)
Dissect out a small piece of mycelium containing nematodes and traps of each type, place on microscope slide and examine under the compound microscope.
3. Diplogasterid nematodes as predators. Pipette prey nematodes in a small amount of water onto Petri dishes with Pristionchus sp. Observe for feeding.
4. Powerpoint presentation: The soil food web.
5. Videotapes:
6. Lab project reports.
7. Term Project (Dear Becky Letter) discussion..
8. Homework Assignment:
NEMAPLEX Exercise
Main Menu:
Select...Management and Decision Support
Select...Antogonists of Nematodes
Review diversity of nematode antagonists.
Select...Nematode Management
Select...Reduction and Eradication: Biological
Review approaches.
Select...Biological Management
Review approaches in biological management of nematodes.
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