NEMATOLOGY 100
Laboratory Exercise 15
Rev. 11/13/2008
Purpose: Sedentary endoparasitic nematodes: morphology, anatomy, biology, feeding habits -- Meloidogyne (Tylenchida, Tylenchina, Tylenchoidea, Heteroderidae).
1. Slide presentation of characteristics.
2. Inspect tomato roots and grape roots infected with Meloidogyne incognita. Note hyperplasia, galling. Examine root surface for presence of egg masses.
3. Examine Meloidogyne incognita juveniles and males extracted from soil.
4. Dissect adult females from the tomato root system provided. Place on a microscope slide in a small drop of lactic acid. Carefully cut the perineal section from the posterior region of the female of each species and mount on a microscope slide for observation under the compound microscope. Also try the "squash and roll" technique.
5. Identify species of Meloidogyne from perineal patterns and protein patterns using the keys provided.
6. Note the taxonomic position of the root-knot nematodes in the order Tylenchida, suborder Tylenchina.
7. Hutchison Hall field trip - Molecular Diagnostics, laboratory of Dr. Steve Nadler.
8. Videotapes
9. Homework Assignment:
NEMAPLEX Exercise
Main Menu:
Select...Biology and Taxonomy
Select..Phylogenetic Classification
Select...Tylenchida
Select...Heteroderidae
Review Meloidogyne, including M. incognita, M. hapla,
M. javanica, and M. arenaria.
Return to Main Menu
10. NEMABASE Exercise