NEMATOLOGY 100

Laboratory Exercise 14

Rev. 11/11/2008

Purpose:  Semi-endoparasitic nematodes:  morphology, anatomy, biology, feeding habits -- Heterodera, Globodera, Punctodera and other cyst nematodes (Tylenchida, Tylenchina, Tylenchoidea, Heteroderidae).

 1.  Slides of characteristics and damage by cyst nematodes.

 2.  Inspect sugarbeet  roots to observe symptoms and specimens of Heterodera schachtii.

 3.  Stain sugarbeet roots with acid-fuchsin/lactic acid; mount root pieces between two slides and distinguish various life stages of the cyst nematode.  Observe the syncytial feeding site.

 4.  Hand-pick males and juveniles of Heterodera schachtii.
      Draw the nematodes observing the following characteristic features where appropriate:

          a)   stylet length and morphology
          b)   esophagus, type and anatomy, nature of postcorpus interface with intestine
          c)   male and female reproductive structures
          d)   general shape when heat-relaxed

5.  Cut off the vulval cone and mount on a slide.  Determine whether the cysts of H. schachtii are bullate or abullate.

6.  Examine the range of shapes of cysts of various species of Heteroderidae.

 7.  Note the taxonomic position of these nematodes in the order Tylenchida, suborder Tylenchina.

 8.  Examine the descriptive charts of these plant-parasitic nematodes.

 9.  Report on Lab Project:

• Complete your nematode identifications.
• Use Nemaplex to complete your faunal analysis:

From Nemaplex Main Menu:

            Select Decision Support

       Select Nematodes as Bioindicators: Faunal Analysis

                        Select Nematode Faunal Analyzer

        Enter your data in the indicated column. 

        What does the analysis suggest about your soil?

10.  Videotapes:

          a)  Behavior of Heterodera species through the Life Cycle.

          b)  The Development of Heterodera schachtii.

          c)  Soybean Cyst Nematode Management.

11.   Homework Assignment:

        NEMAPLEX Exercise
          Main Menu:
               Select...Biology and Taxonomy
                Select..Phylogenetic Classification
                 Select...Tylenchida
               Select...Heteroderidae
                           Review Heterodera, including H. schachtii,  
                           Review Globodera, including G. rostochiensis,  and G. pallida.  
          

12.  NEMABASE Exercise

           Main Menu
            Select Decision Support

                       Select Host Status Database

 

Determine the host range for the genera Heterodera and Globodera.

In Nemabase select "All Plants" and "Genus" Heterodera and Globodera. 

Also examine the host range of individual species.
Set "Host" filter to Host.  Process.

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