Laboratory Exercise 14
Purpose: Semi-endoparasitic nematodes: morphology, anatomy, biology, feeding habits -- Heterodera, Globodera, Punctodera and other cyst nematodes (Order Rhabditida, Suborder Tylenchina, Tylenchoidea, Heteroderidae).
1. Powerpoint presentation and slides of characteristics and damage by cyst nematodes.
2. Inspect Brussels Sprouts roots to observe symptoms and specimens of Heterodera sp.
3. Stain Brussels Sprouts roots with acid-fuchsin/lactic acid (see Nemaplex Methods section); mount root pieces between two slides and distinguish various life stages of the cyst nematode. Observe the syncytial feeding site.
4. Hand-pick males and juveniles of Heterodera
sp. from Brussels Sprouts,
Draw the nematodes observing the following characteristic features where appropriate:
a) stylet length and morphology
b) esophagus, type and anatomy, nature of postcorpus interface with intestine
c) male and female reproductive structures
d) general shape when heat-relaxed
5. Cut off the vulval cone and mount on a slide. Determine whether the cysts are bullate or abullate. Determine whether this nematode is Heterodera schachtii or H. cruciferae.
6. Examine the range of shapes of cysts of various species of Heteroderidae.
7. Note the taxonomic position of these nematodes in the Order Rhabditida, Suborder Tylenchina.
8. Examine the descriptive charts of these plant-parasitic nematodes.
9. Anhydrobiosis Experiment (continued):
11. Homework Assignment:
Select...Biology and Taxonomy
Select...Rhabditida then Tylenchina
Review Heterodera, including H. schachtii,
Review Globodera, including G. rostochiensis, and G. pallida.
12. NEMABASE Exercise
Review the host range of
nematodes in the genera Heterodera and Globodera. What are the
indicated non-hosts? Are there resistant cultivars available?
From the Nemaplex main Menu, select Management and Decision Support, then Nemabase 2012.
Alternatively, you can access the data from the descriptive page for each nematode.
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