NEMATOLOGY 100
Laboratory Exercise 9
Rev. 10/28/2008
Purpose: Migratory endoparasitic nematodes: morphology, anatomy, biology, feeding habits -- Pratylenchidae (Tylenchida, Tylenchoidea)
1. Slide presentation of characteristics.
2. Hand-pick live adults of the specimens of Pratylenchus neglectus or P. vulnus, Hirschmanniella pomponiensis (?) and preserved specimens of Radopholus similis provided.
Draw the nematodes, observing the following characteristic features:
a) stylet length and morphology.
b) esophagus, type and anatomy, nature of
postcorpus interface with intestine.
c) male and female reproductive structures.
d) general shape when heat-relaxed.
3. Note the taxonomic position of these nematodes in the order Tylenchida.
4. Examine descriptive charts of these plant-parasitic nematodes in the Pratylenchidae, especially those of Pratylenchus, Radopholus and Hirschmanniella.
5. Videotapes:
a) The Burrowing Nematode
b) Nematode predation and
ingestion by Labronema species (Dorylaimida)
and
Mononchus aquaticus (Mononchida).
7. Study photographs of characteristics and damage by migratory endoparasites: Radopholus and Hirschmanniella
8. Powerpoint presentation: Nematodes as Bioindicators.
Lab Project extractions should be started, analyses will be performed in next two lab periods. Please see details on Lab Exercise 10 outline.
9. Report on your assessment of the functional status of the soil food web suggested by the nematode assemblage in:
10. Homework Assignment:
NEMAPLEX Exercise
Main Menu:
Select...Biology and Taxonomy
Select..Phylogenetic Classification
Select...Tylenchida
Select...Pratylenchidae
Review Pratylenchus including some of the species.
Review Radopholus including R. similis.
Review Hirschmanniella including H. oryzae and
H. belli.
Return to Main Menu
10. NEMABASE Exercise
Determine the host range for these genera. In Nemabase select "All Plants" and "Genus" Radopholus, then Hirschmanniella,
Hoplolaimus and Scutellonema. Set "Host" filter to Host. Process.