NEMATOLOGY 100
Laboratory Exercise 7
Rev. 10/16/2008
Purpose: Ectoparasitic nematodes: Orders Dorylaimida and Triplonchida
Morphology, anatomy,
feeding habits:
Longidoridae
(Dorylaimoidea, Dorylaimina,
Dorylaimida)
Trichodoridae
(Diphtherophoroidea, Diphtherophorina,
Triplonchida)
1. Slide presentation of characteristics and virus transmission.
2. Hand-pick live adults of
Draw the nematodes, observing the following characteristic features:
a) spear length and morphology.
b) esophagus
type and anatomy, nature of postcorpus
interface with intestine.
c) male and
female reproductive structures.
d) general
shape when heat-relaxed.
3. Examine prepared slides of Longidorus and Xiphinema. Note differences in odontostyle and odontophore.
4. Note the taxonomic position of these nematodes in the orders Dorylaimida and Triplonchida.
5. Examine descriptive charts of these plant-parasitic nematodes in the Dorylaimida and Triplonchida
6. Examine nematode communities of student samples.
7. Videotape: Xiphinema index feeding on roots of seedlings.
8. Complete the field trip sampling study. Report to the class your conclusions about the reliability of the sampling strategies we used for measuring nematode populations in alfalfa.
9. Study photographs of characteristics and damage by migratory ectoparasites: Xiphinema, Longidorus and Trichodorus. Also, slides of the ultrastructure of these nematodes
10. Homework Assignment:
NEMAPLEX Exercise
Main Menu:
Select...Biology and Taxonomy
Select..Phylogenetic Classification
Select...Order Dorylaimida
Review characteristics of this Order.
Select...Longidoridae
Review Longidorus, Paralongidorus, Xiphinema,
including some of the species.
Return to Classification Menu
Select...Order Triplonchida
Review characteristics of this Order.
Select...Trichodoridae
Review Trichodorus, Paratrichodorus,
including some of the species.
Return to
Main Menu
Select...General Nematology
Select...Nematodes as Plant Parasites
Select...Nematodes as Virus Vectors
Return to
Main Menu
11. Nemabase Exercise
Review the host range of
these nematodes. What are the indicated non-hosts?
In Nemabase select
"All Plants" and "Genus" to the genus of interest.
Set "Host"
filter to Host. Process.