NEMATOLOGY 100

Laboratory Exercise 3

Rev. 10/03/2008

Purpose:  Techniques for Extraction of Nematodes from Soil and Plant Tissue:  Qualitative and Quantitative Population Assessment

     1.   Videotape and discussion of separation practices.

     2.   Slides on separation efficiency.

     3.   Demonstration of techniques for separating nematodes from soil and roots and population assessment.
       

          a)   Techniques requiring nematode movement.

               1)   Baermann funnel and modifications.
               2)   Mist chamber.

          b)   Flotation techniques.

               1)   Cobb's decanting and sieving.
               2)   Seinhorst two-flask method.
               3)   Single and multiple elutriators.
               4)   Sugar flotation/centrifugation.

     4.   Symptom evaluation and bioassay.

          a)   Root staining.
          b)   Gall rating.

    5.  Demonstration of UCR Virtual Nematology CD.

    6.  Examination of samples extracted in this laboratory period:

a)   Draw the female reproductive tract; indicate whether the arrangement of uteri is 
       prodelphic, opisthodelphic, amphidelphic.

b)   Draw representative male reproductive structures,   	including spicules and gubernaculum, caudal alae.

    7.  Homework Assignment:

         Review sections of NEMAPLEX on the higher classification of the phylum Nematoda as follows:

           Main Menu:
              Select...Biology and Taxonomy
                            Select...Phylum Nematoda
                               Select...Higher Classification of Nematodes
                                     Class Secernentea
                                     Class Adenophorea
           Main Menu:
              Select...General Nematology
                         Select...Nematodes as Plant Parasites
                            Select...Taxa containing Plant Parasites

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