Xiphinema americanum

 

Contents

 

Rev 12/27/2013

 Dagger Nematode Classification Hosts
Morphology and Anatomy Life Cycle
Return to Xiphinema Menu Economic Importance Damage
Distribution Management
Return to Longidoridae Menu Feeding  References
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 Classification:

    Enoplea
      Dorylaimida
       Dorylaimina
        Dorylaimoidea
         Longidoridae
          Xiphineminae

            Xiphinema americanum Cobb, 1913

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Morphology and Anatomy:

  Note odontostyle, guiding ring, flanged odontophore, odontostyle/odontophore junction 
Male:

Has same general configuration as female.  Actually, males of X. americanum are seldom found.

Note diorchic testes, with one branch anterior and one branch posterior, around mid body.  Connected to cloaca through seminal vesicle and vas deferens.

Female:

Vulva region and tail region.

Female is diovarial and amphidelphic.

Vulva at 46-54% of body length.

Xiphinema americanum is actually considered to be a group of some 39 species, which include X. brevicolle and X. pachtaicum.  

Molecular techniques are further underscoring the variability within the group and adding to the body of knowledge that will be required for an eventual taxonomic revision (Vrain, 1993).  

The group also includes X. californicum which is probably associated with grape in California (Lamberti and Ciancio, 1993).

Xiphinema americanum group.  Adult stages of this nematode range in length from 1.3 to 2.2 mm. However, the X. americanum sensu stricto ranges from 1.6 to 1.8 mm in length with an odontostyle and odontophore length slightly greater then 100 m . 

The morphometrics of these species tend to overlap (Robbins and Brown, 1991). 

 

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Distribution:

It is currently suggested that the true X. americanum is native to, and predominant in, the eastern U.S. whereas the western U.S. populations are actually X. californicum, X. pacificum and other species (Lamberti and Bleve-Zacheo, 1979). Although true X. americancum occurs in California it may not be as common as X. californicum.

Members of the X. americanum group are common throughout vineyard areas of California, with the notable exception of the drier and warmer Coachella Valley (Siddiqui et al., 1973). They are most numerous in vineyards with abundant other vegetation, usually those in areas with >45 cm annual rainfall.

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Economic Importance:

 

C-rated pests in California.  (See also Xiphinema californicum.)


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Feeding:

Migratory root ectoparasite; all stages feed at root tips.  

Deep penetration of root tip by stylet; causes hypertrophy of cells, wall thickening, etc.   

  Causes necrosis of feeder roots on strawberries.
    

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Hosts:

Species in this group of nematodes are difficult to study biologically because they are not readily cultured in greenhouse or laboratory conditions. They have a wide host range that includes common weeds and grasses, strawberries, and soybeans, forest trees (spruce, pine, etc.), perennial orchards, grape.  90% of California pear orchards (French, 1964).

The breadth of the host range is probably also related to the genetic diversity within the species complex.

For an extensive list of host plant species and their susceptibility, copy the name

Xiphinema americanum

select Nemabase and paste the name in the Genus and species box


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Life Cycle:

Four larval stages were thought to occur in the soil as there is no molt in the egg.  However, recent studies indicate that some members of the X. americanum group (including X. americanum sensu stricto and X. californicum) pass through only three juvenile stages before becoming adults (Halbrendt and Brown, 1992).  

Molting does not occur in the egg and the first stage hatches.

The length of the life cycle has not been experimentally determined; speculated to be 1 year (Malek, 1969).  However, two population peaks are sometimes observed per year, although not in our (Ferris and McKenry, 1974) studies in California.  Current conventional wisdom is that the longevity of X. americanum individuals is  3 to 5 years, with only one generation produced per year (Halbrendt and Brown, 1993). 

In field situations, this nematode is sensitive to lack of oxygen, as are many of the larger nematodes. Their shallow occurrence in soil, and preference for less disturbed sites within the vineyard, results in their greatest abundance in the undisturbed soil in the vine row (Ferris and McKenry, 1974).

Research experience and conventional wisdom has been that X. americanum is difficult to culture in pots under greenhouse conditions (Cohn and Mordechai, 1969, Lownsbery and Mitchell, 1965), which may have deterred researchers from attempting to work with the nematode under controlled conditions.  The earlier work suggested that maintenance of relatively constant moisture conditions was important (Lownsbery and Mitchell, 1964) and more recent studies suggest that regulated temperature conditions are favorable for the nematode and that sorghum and grasses are useful greenhouse hosts (Diop et al., 2001). The temperature and moisture conclusions are quite interesting because, under field conditions in California vineyards, X. americanum is most prevalent in the upper 30-45 cm of soil where aeration conditions are favorable but soil moisture and temperature conditions fluctuate the most (Ferris and McKenry, 1974). 

 

Distribution patterns of Xiphinema americanum remained remarkably similar in a 2-ha field near Pixley, California, during the course of a year in a field in a cowpea/cotton rotation.  The difference in height of the peaks in the two graphs indicates differences in host status between cotton and cowpea and their associated weeds.
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Damage:

Nematode causes root stunting and tip galling.  

It causes necrosis on strawberry roots.  It is also common in declining vineyards at high population densities.  Marked growth response in grapevines were obtained after applications of DBCP.  

From field observations, certain populations identified as X. californicum can build to 4-5 times the population level of a typical X. americanum sensu stricto. Population levels of X. americanum sensu lato in excess of 500 actual individuals/250 cm3 in non-weedy sites should be suspected as a probable cause for vine damage (McKenry, 1992).

Xiphinema americanum is a NEPO virus vector - polyhedral particles.

Xiphinema americanum sensu lato vectors:

Populations within the X. americanum group are capable of transmitting various strains of tomato ringspot virus (TRSV):

However, some populations keyed to the same species may not be able to transmit the virus (Brown et al., 1993).

Virus particles can be acquired within 24 hours of exposure to root.  They occur in the esophageal lumen near stylet extension and in the glandular region of the esophagus.

In California, TRSV strains have not posed serious production problems in grape vineyards like those due to grapevine fanleaf virus. This could be a direct result of quarantines on virus infected grape cultivars. Vine damage attributable to the nematode alone is not great. At high inoculum levels, there was significant damage to newly planted 'French Colombard' in one experiment but not in others. Damage may occur when other factors also reduce root systems or force a shallow root system (Ferris and McKenry, 1975). 

 

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Management:

Some conventional grape rootstocks tested by McKenry in California have shown some resistance to X. americanum sensu stricto include 'VR-O39-16', 'Freedom', 'Dog Ridge', and 'Schwarzmann' (McKenry and Kretsch, 1994). 

In contrast, V. vinifera and '1613C' are good hosts. In vineyards, 'VR-O39-16' and 'Freedom' do build-up populations of X. americanum sensu lato, however.

Resistance

For a list of plant species or cultivars (if any) reported to be immune or to have some level of resistance to this nematode species, copy the name

Xiphinema americanum

select Nemabase Resistance Search and paste the name in the Genus and species box

 

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References:

CIH Descriptions of Plant-parasitic Nematodes, Set 2, No. 29 (1973).

Cohn E., and Mordechai, M. 1969. Investigations on the life cycles and host preference of some species of Xiphinema and Longidorus under controlled conditions. Nematologica 15:295-302.

Diop, M.T., Dim, J-H., Mountport, D. and Baujard, P. 2001. Laboratory culture of two Xiphinema americanum-group species (Nematoda: Longidoridae) from Senegal. Nematology 3:411-415.

Ferris, H. and M. V. McKenry.  1974.  Seasonal fluctuations in the spatial distribution of nematode populations in a California vineyard.  Journal of Nematology 6:203-210.

Lownsbery, B.F. and Mitchell, J.T. 1965. Some effects of chemical amendments and cultural conditions on population levels of Xiphinema americanum. Pl. Dis. Reptr 49, 994-998.

 

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Copyright 1999 by Howard Ferris.
Revised: December 27, 2013.